Nucleic Acids: OD260 Convertor

This tool will convert OD260 absorbance to μg/ml concentration.

Sample OD at 260nm

Sample Type




Oligo:
Concentration
{{result.conc}} μg/ml
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Formula
concentration (ug/ml) = OD260 x conversion factor
conversion factors:
  • 1 OD260 Unit = 50 μg/ml for dsDNA
  • 1 OD260 Unit = 40 μg/ml ssRNA
  • 1 OD260 Unit = 33 μg/ml ssDNA
ss oligo concentration (ug/ml) = OD260 x MW x 1000 / ε260
where MW = molecular weight of oligo (non-phosphorylated)
and ε260 = extinction coefficient of oligo at 260nm